Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (1): 41-45.doi: 10.3969/j.issn.1673-8225.2011. 01.009

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Bone marrow mesenchymal stem cells for the treatment of immune induced aplastic anemia

Huang Ying1, Zheng Cui-ling2, Yang Shao-guang3, Han Zhong-chao3   

  1. 1Department of Hematology, First Affiliated Hospital, Kunming Medical College, Kunming  650031, Yunnan Province, China
    2Department of Clinical Laboratory, Tumor Hospital, Chinese Academy of Medical Sciences, Beijing  100021, China
    3Key Laboratory, Tianjin Institute of Hematology, Chinese Academy of Medical Sciences, Tianjin  300020, China
  • Received:2010-08-05 Revised:2010-10-26 Online:2011-01-01 Published:2011-01-01
  • About author:Huang Ying☆, Doctor, Lecturer, Department of Hematology, First Affiliated Hospital, Kunming Medical College, Kunming 650031, Yunnan Province, China jjhy77@yahoo.com.cn
  • Supported by:

    the Science and Technology Project of Yunnan Province, No. 2008ZC134M

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Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) have immunomodulatory effects on lymphocytes and other immune-associated cells. Supportive effects of BMSCs on hematopoietic cells had been certified. Occurrence and development of aplastic anemia are closely associated with T cell hyperfunction-induced hematopoietic stem/progenitor cells.
OBJECTIVE: To compare the therapeutic effects of BMSCs and whole bone marrow cells in immune-induced aplastic anemia mice.
METHODS: Immune-induced aplastic anemia BALB/C mouse models were established. The BALB/C mice were randomly assigned to normal, control, BMSC injection and whole bone marrow cell groups. Therapeutic effects of BMSCs and whole bone marrow cells, volume of hemopoietic tissues, adipocytes and blood sinus structure were observed.
RESULTS AND CONCLUSION: ①On day 20, all mice without treatment (8/8) and 4 of 8 mice which received BMSCs died, while 7 of 8 mice were infused with bone marrow cells and kept live. ②No significant difference in change of weight, blood cells, haematopoiesis was detected between BMSCs or bone marrow cells. ③In spleen cells of improved mice with BMSCs or bone marrow cells, the percentages of CD4+T cells, CD8+T cells and the ratio of CD4+/CD8+ were similar. In peripheral blood, the percentages of CD4+T cells and CD8+T cells were lower in BMSCs group than in normal group. The level of CD8+T cells was lowest in mice with bone marrow cells. These results indicate that BMSCs improve aplastic anemia symptom and recover hematopoiesis. The bone marrow cells were more effective than BMSCs in the therapeutical effect, but no significant difference was determined.

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